Vitrification of mouse MII oocytes: Developmental competency using paclitaxel

Closed vitrification of mouse oocytes using the CryoLogic vitrification method: A modification that improves developmental competence

OBJECTIVE To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM) were tested. METHODS Mature oocytes obtained from female BDF-1 mice were vitrified by two-step exposure to equilibrium and vitrification solutions. Three vitrification protocols were tested on...

P-66: The Effect of Vitrification on Distribution of ?9, ?v, ?1, ?3 Integrins Mouse Oocytes at GV, IVM-MII and OV- MII Stages

Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability

High survival of mouse oocytes using an optimized vitrification protocol

The method of vitrification has been widely used for cryopreservation. However, the effectiveness of this method for mammalian oocytes could be improved by optimizing each step of the process. In the present study, we tested the effects of varying several key parameters to determine the most effective protocol for mouse oocyte vitrification. We found that cryoprotectant containing ethylene glyc...

P-132: The Effect of Vitrification on Mouse Oocytes Survivale Rate and Apoptosis Using Cryotop

Background: Vitrification of oocyte is one of the most important topics in the field of assisted reproductive techniques (ART). Considering the importance of oocyte vitrification in clinics, in the present study the effect of vitrification on mouse oocytes survivale rate and apoptosis by cryotop were investigated. Materials and Methods: 200 GV oocytes and 200 MII oocytes were obtained respectiv...